1) In this case the the Pub Manager Access Denied error was occurring because the (ChainTrust) web.config certificate validation was failing to find the root certificate in the trusted root store. This was proved by reducing the scope of the certificate validation to (PeerTrust), by changing the following line in .\InfoShare\WebDITA\InfoShareWS\Web.config:
<certificateValidation certificateValidationMode="ChainTrust"/>
to
<certificateValidation certificateValidationMode="PeerTrust"/>
So either the root certificate can be added to the trusted store or since it's a test environment the above change may be sufficient.
2) Also can be the timezone or clock is out by more than 5 minutes and the tokens expire before getting used.
3) This can also be fixed by locking client desktop screen while being on VPN connection and than unlocking it.
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